Replacement of the murine leukemia virus (MLV) envelope gene with a truncated HIV envelope gene in MLV generates a virus with impaired replication capacity

Murine Leukemia Virus (A-MLV)

Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent.

BACKGROUND The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients. METHODS We wanted to establish a MLV-related virus real-time PCR for routine diagnostics. RESULTS Our study identified false positive ML...

Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells (gene therapyyenv geneytransmembrane protein truncation)

CD4-expressing T cells in lymphoid organs are infected by the primary strains of HIV and represent one of the main sources of virus replication. Gene therapy strategies are being developed that allow the transfer of exogenous genes into CD41 T lymphocytes whose expression might prevent viral infection or replication. Insights into the mechanisms that govern virus entry into the target cells can...

Sequences and Phylogenetic Analysis of Envelope (E) Gene of Iranian Infectious Bronchitis Virus Isolates in Iran

Background and Aims: Infectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis (IB), which is an acute and highly contagious disease of the respiratory and sometimes the urogenital tracts of chickens. In Iran, the disease has been identified in chicken flocks with serological and virus isolation methods. Gene 3c (E) encode envelop (small membrane) protein. For envelope...

Mutations affecting the MA portion of the v-Abl protein reveal a conserved role of Gag in Abelson murine leukemia virus (MLV) and Moloney MLV.

Abelson murine leukemia virus (Ab-MLV) arose from a recombination between gag sequences in Moloney MLV (Mo-MLV) and the c-abl proto-oncogene. The v-Abl oncoprotein encoded by Ab-MLV contains MA, p12, and a portion of CA sequences derived from the gag gene of Mo-MLV. Previous studies indicated that alteration of MA sequences affects the biology of Mo-MLV and Ab-MLV. To understand the role of the...